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Sample GSM1309419 Query DataSets for GSM1309419
Status Public on Jan 18, 2014
Title differentiated H1 hESC biological rep 1
Sample type RNA
 
Source name H1 hESC differentiated to EB for 3days
Organism Homo sapiens
Characteristics cell line: H1
cell type: human embryonic stem cells (hESCs)
cell status: differentiated to EB for 3days
Growth protocol hESCs were cultured in mTeSR1 medium for undifferentiated cells. For EB formation, cells were dissociated with Accutase (Stem Cell Technologies, Vancouver, Canada) and plated on ultra low attachment plates (Corning Life Science, Tewksbury, MA) in hESGM (without bFGF) with 10 μM Y-27632 (Chemdea, Ridgewood, NJ) for designated period of time.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridazation was performed according to standard protocol recommended by Affymetrix.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description H1_EB_1
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jan 17, 2014
Last update date Jan 18, 2014
Contact name Yong Kim
E-mail(s) thadyk@ucla.edu
Organization name UCLA
Street address 10833 Le Conte Ave, 73-022 CHS
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL570
Series (1)
GSE54186 Discovery of consensus gene signature and intermodular connectivity defining self-renewal of human embryonic stem cells

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 3391.498
1053_at 1170.157
117_at 59.65297
121_at 714.0543
1255_g_at 417.6112
1294_at 75.08539
1316_at 172.954
1320_at 52.80957
1405_i_at 28.98292
1431_at 47.09338
1438_at 234.7578
1487_at 380.1848
1494_f_at 82.31525
1552256_a_at 1019.331
1552257_a_at 1610.316
1552258_at 12.05816
1552261_at 46.44226
1552263_at 587.739
1552264_a_at 392.5115
1552266_at 30.63761

Total number of rows: 54675

Table truncated, full table size 1052 Kbytes.




Supplementary file Size Download File type/resource
GSM1309419_H1_EB_1.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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