|
| Status |
Public on Jan 18, 2014 |
| Title |
differentiated H1 hESC biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
H1 hESC differentiated to EB for 3days
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H1
cell type: human embryonic stem cells (hESCs)
cell status: differentiated to EB for 3days
|
| Growth protocol |
hESCs were cultured in mTeSR1 medium for undifferentiated cells. For EB formation, cells were dissociated with Accutase (Stem Cell Technologies, Vancouver, Canada) and plated on ultra low attachment plates (Corning Life Science, Tewksbury, MA) in hESGM (without bFGF) with 10 μM Y-27632 (Chemdea, Ridgewood, NJ) for designated period of time.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridazation was performed according to standard protocol recommended by Affymetrix.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
H1_EB_2
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 17, 2014 |
| Last update date |
Jan 18, 2014 |
| Contact name |
Yong Kim |
| E-mail(s) |
thadyk@ucla.edu
|
| Organization name |
UCLA
|
| Street address |
10833 Le Conte Ave, 73-022 CHS
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE54186 |
Discovery of consensus gene signature and intermodular connectivity defining self-renewal of human embryonic stem cells |
|
