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Sample GSM1309421 Query DataSets for GSM1309421
Status Public on Jan 18, 2014
Title undifferentiated H9 hESC biological rep 1
Sample type RNA
 
Source name H9 hESC undifferentiated
Organism Homo sapiens
Characteristics cell line: H9
cell type: human embryonic stem cells (hESCs)
cell status: undifferentiated
Growth protocol hESCs were cultured in mTeSR1 medium for undifferentiated cells. For EB formation, cells were dissociated with Accutase (Stem Cell Technologies, Vancouver, Canada) and plated on ultra low attachment plates (Corning Life Science, Tewksbury, MA) in hESGM (without bFGF) with 10 μM Y-27632 (Chemdea, Ridgewood, NJ) for designated period of time.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridazation was performed according to standard protocol recommended by Affymetrix.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description H9_und_1
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jan 17, 2014
Last update date Jan 18, 2014
Contact name Yong Kim
E-mail(s) thadyk@ucla.edu
Organization name UCLA
Street address 10833 Le Conte Ave, 73-022 CHS
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL570
Series (1)
GSE54186 Discovery of consensus gene signature and intermodular connectivity defining self-renewal of human embryonic stem cells

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 2843.113
1053_at 1325.607
117_at 75.67853
121_at 635.595
1255_g_at 1188.786
1294_at 79.14674
1316_at 118.5072
1320_at 38.78284
1405_i_at 6.584849
1431_at 26.6707
1438_at 25.72219
1487_at 576.0497
1494_f_at 108.0478
1552256_a_at 1168.606
1552257_a_at 3071.361
1552258_at 45.50868
1552261_at 50.8516
1552263_at 980.504
1552264_a_at 629.7092
1552266_at 21.73099

Total number of rows: 54675

Table truncated, full table size 1052 Kbytes.




Supplementary file Size Download File type/resource
GSM1309421_H9_und_1.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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