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Sample GSM1309422 Query DataSets for GSM1309422
Status Public on Jan 18, 2014
Title undifferentiated H9 hESC biological rep 2
Sample type RNA
 
Source name H9 hESC undifferentiated
Organism Homo sapiens
Characteristics cell line: H9
cell type: human embryonic stem cells (hESCs)
cell status: undifferentiated
Growth protocol hESCs were cultured in mTeSR1 medium for undifferentiated cells. For EB formation, cells were dissociated with Accutase (Stem Cell Technologies, Vancouver, Canada) and plated on ultra low attachment plates (Corning Life Science, Tewksbury, MA) in hESGM (without bFGF) with 10 μM Y-27632 (Chemdea, Ridgewood, NJ) for designated period of time.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridazation was performed according to standard protocol recommended by Affymetrix.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description H9_und_2
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jan 17, 2014
Last update date Jan 18, 2014
Contact name Yong Kim
E-mail(s) thadyk@ucla.edu
Organization name UCLA
Street address 10833 Le Conte Ave, 73-022 CHS
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL570
Series (1)
GSE54186 Discovery of consensus gene signature and intermodular connectivity defining self-renewal of human embryonic stem cells

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 2674.343
1053_at 1453.451
117_at 75.86109
121_at 398.1948
1255_g_at 1128.059
1294_at 76.6657
1316_at 106.6443
1320_at 26.64166
1405_i_at 0.7410975
1431_at 27.07815
1438_at 35.32295
1487_at 618.3544
1494_f_at 102.5458
1552256_a_at 1107.969
1552257_a_at 2969.266
1552258_at 6.819582
1552261_at 34.30289
1552263_at 881.9998
1552264_a_at 590.1113
1552266_at 8.40128

Total number of rows: 54675

Table truncated, full table size 1052 Kbytes.




Supplementary file Size Download File type/resource
GSM1309422_H9_und_2.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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