|
| Status |
Public on Nov 21, 2014 |
| Title |
Liver from SMRT and NCoR double knock out mice, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Liver_SMRT and NCoR double knock out
|
| Organism |
Mus musculus |
| Characteristics |
strain background: 129SV/B6 mixed
genotype/variation: SMRT and NCoR KO
gender: female
age: 7-9 weeks
tissue: liver
|
| Treatment protocol |
At 7-9 weeks of age on standard chow (Harlan Teklad F6 Rodent Diet 8664) mice were sacrificed and RNA isolated.
|
| Growth protocol |
Mice were housed with a 12 hour light/dark cycle and supplied with food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Perfect Pure RNA tissue kit (5 Prime)
|
| Label |
biotin
|
| Label protocol |
200ng of total RNA were amplified and labeled using the Ambion Whole Transcript Expression Kit (Catalog Number 4425209, Ambion, Austin, TX) and Affymetix Genechip Whole Transcript Terminal Labeling Kit (Catalog Number 900671, Affymetrix, Santa Clara, CA)
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip® Mouse Gene 2.0 ST arrays were washed, stained and scanned according to the protocol described in GeneChip® WT Terminal Labeling and Hybridization User Manual (Fluidics Protocol FS450_0002)
|
| Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 3000 7G, software AGCC 3.1.1
|
| Description |
DKO 184
|
| Data processing |
RMA Signal extraction, normalization and filtering was performed as described using the comprehensive R- and bioconductor-based web service for microarray data analysis – ‘CARMAWeb’
|
| |
|
| Submission date |
Jan 17, 2014 |
| Last update date |
Nov 22, 2014 |
| Contact name |
Markus Jeitler |
| E-mail(s) |
markus.jeitler@meduniwien.ac.at
|
| Organization name |
Medical University Vienna
|
| Street address |
Lazarettgasse 14
|
| City |
Vienna |
| ZIP/Postal code |
A-1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE54192 |
NCoR1 and SMRT play unique roles in thyroid hormone signaling in the liver |
|
