|
| Status |
Public on Mar 19, 2014 |
| Title |
shRen;p53-/- HSCPC Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
shRen;p53-/- HSCPC
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
transplanted with: shRen;p53-/-
time point: 6 wks after transplant
cell type: sorted lin-ckit+ hematopoietic stem and progenitor cells (HSCPCs)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed and RNA extracted with Trizol reagent in accordance with the prescribed protocol provided by the manufacturer. Quality control was performed with Agilent Bioanalyser and Nanodrop.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for Illumina GX mouse-ref8 v2 R3 Array. Slides were washed and stained in the Illumina instrument.
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Ren_1
|
| Data processing |
The data were median centered using R
|
| |
|
| Submission date |
Jan 22, 2014 |
| Last update date |
Mar 19, 2014 |
| Contact name |
Chong Chen |
| E-mail(s) |
chen_chong@yahoo.com
|
| Organization name |
Sichuan University
|
| Department |
State Key Laboratory of Biotherapy
|
| Lab |
Chong Chen
|
| Street address |
17 Renmin S. Road-No. 3
|
| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610041 |
| Country |
China |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE54313 |
Expression analysis of Mll3 knockdown p53-/- HSPC vs. control p53-/- HSPC |
|
