|
| Status |
Public on Mar 01, 2014 |
| Title |
Heart_KO_Rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Heart_KO
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6J
gender: female
age: 20 wks
genotype/variation: Nelf-b f/f; CreER
tissue: heart
|
| Treatment protocol |
fasting for 5 hours
|
| Growth protocol |
Regular Chow
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy mini kit (Qiagen, Cat. #217004)
|
| Label |
Biotin
|
| Label protocol |
TotalPrepTM RNA amplification kit (Ambion, Cat. #AMIL1791)
|
| |
|
| Hybridization protocol |
MouseRef-8 version 2.0, Illumina
|
| Scan protocol |
BeadChips were scanned on a BeadArrayTM Reader (Illumina) using BeadScan software (version 3.2, Illumina)
|
| Data processing |
the scanned data were uploaded into GenomeStudio® software (version 1.1, Illumina) via the gene expression module (Direct Hyb).
|
| |
|
| Submission date |
Jan 24, 2014 |
| Last update date |
Mar 01, 2014 |
| Contact name |
Haihui Pan |
| E-mail(s) |
panh@livemail.uthscsa.edu
|
| Phone |
210-562-4159
|
| Organization name |
University of Texas Health Science Center at San Antonio
|
| Department |
Molecular Medicine
|
| Lab |
Dr. Rong Li
|
| Street address |
8403 Floyd Curl Dr.
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE54372 |
Heart tissue from Nelf-b CreER mice |
|
