|
Status |
Public on Jun 09, 2014 |
Title |
Kidney_WT_rep3 |
Sample type |
RNA |
|
|
Source name |
Kidney_WT
|
Organism |
Mus musculus |
Characteristics |
strain background: C57BL/6 N genotype/variation: Raptor fl/fl developmental stage: P14 tissue: Kidney
|
Treatment protocol |
Kidneys were split in half and immediately snap frozen in liquid nitrogen.
|
Growth protocol |
Raptor fl/fl*KspCre and Raptor fl/fl animals were sacrificed at P14 before the development of an overt functional phenotype
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Mini Plus Kit (Qiagen, Hilden, Germany)
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA was generated from 1.5 µg total RNA using the One-Cycle Target Labeling Assay (Affymetrix).
|
|
|
Hybridization protocol |
15 µg labelled cRNA were hybridized onto Mouse 4302 arrays (Affymetrix, CA, USA) for 16h at 45°C using Hybridization Wash and Stain Kit (Affymetrix)
|
Scan protocol |
Arrays were washed and stained with GeneChip Fluidics Station 450 and scanned with GeneChip Scanner 3000 using the GeneChip Operating Software (Affymetrix).
|
Data processing |
R/Bioconductor using Single-Channel Array Normalization (SCAN), (Picolo et al., Genomics 2012).
|
|
|
Submission date |
Jan 27, 2014 |
Last update date |
Jun 09, 2014 |
Contact name |
Hauke Busch |
E-mail(s) |
hauke.busch@uni-luebeck.de
|
Phone |
+49-451-3101-8470
|
Organization name |
University of Lübeck
|
Department |
Lübeck Institute of Experimental Dermatology
|
Street address |
Ratzeburger Allee 160
|
City |
Lübeck |
State/province |
Schleswig-Holstein |
ZIP/Postal code |
23538 |
Country |
Germany |
|
|
Platform ID |
GPL18223 |
Series (1) |
GSE54417 |
mTORC1 maintains renal tubular homeostasis and is essential in response to ischemic stress |
|