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Sample GSM1314722 Query DataSets for GSM1314722
Status Public on Jun 09, 2014
Title Kidney_WT_rep3
Sample type RNA
 
Source name Kidney_WT
Organism Mus musculus
Characteristics strain background: C57BL/6 N
genotype/variation: Raptor fl/fl
developmental stage: P14
tissue: Kidney
Treatment protocol Kidneys were split in half and immediately snap frozen in liquid nitrogen.
Growth protocol Raptor fl/fl*KspCre and Raptor fl/fl animals were sacrificed at P14 before the development of an overt functional phenotype
Extracted molecule total RNA
Extraction protocol RNeasy Mini Plus Kit (Qiagen, Hilden, Germany)
Label Biotin
Label protocol Biotinylated cRNA was generated from 1.5 µg total RNA using the One-Cycle Target Labeling Assay (Affymetrix).
 
Hybridization protocol 15 µg labelled cRNA were hybridized onto Mouse 4302 arrays (Affymetrix, CA, USA) for 16h at 45°C using Hybridization Wash and Stain Kit (Affymetrix)
Scan protocol Arrays were washed and stained with GeneChip Fluidics Station 450 and scanned with GeneChip Scanner 3000 using the GeneChip Operating Software (Affymetrix).
Data processing R/Bioconductor using Single-Channel Array Normalization (SCAN), (Picolo et al., Genomics 2012).
 
Submission date Jan 27, 2014
Last update date Jun 09, 2014
Contact name Hauke Busch
E-mail(s) hauke.busch@uni-luebeck.de
Phone +49-451-3101-8470
Organization name University of Lübeck
Department Lübeck Institute of Experimental Dermatology
Street address Ratzeburger Allee 160
City Lübeck
State/province Schleswig-Holstein
ZIP/Postal code 23538
Country Germany
 
Platform ID GPL18223
Series (1)
GSE54417 mTORC1 maintains renal tubular homeostasis and is essential in response to ischemic stress

Data table header descriptions
ID_REF
VALUE SCAN signal

Data table
ID_REF VALUE
100008567_at 0.554430238
100009600_at -0.05334215
100012_at -0.26313344
100017_at 0.568997812
100019_at 0.681267497
100034251_at 0.183219216
100034739_at -0.208587365
100034748_at 0.753543283
100036520_at -0.034739724
100036521_at 1.650103696
100036523_at 0.009345622
100036768_at 0.009644802
100037258_at 1.529655437
100037262_at -0.046437346
100037278_at 0.253215102
100038347_at 1.013664978
100038358_at -0.153072677
100038371_at -0.179178014
100038392_at -0.13856713
100038398_at 1.184988561

Total number of rows: 17564

Table truncated, full table size 370 Kbytes.




Supplementary file Size Download File type/resource
GSM1314722_Kidney_WT_3.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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