|
| Status |
Public on Aug 08, 2018 |
| Title |
5-CBAxB6-TGFbeta |
| Sample type |
RNA |
| |
|
| Source name |
CBAxB6-TGFbeta
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: CBAxB6-TGFß (KO)
disease state: induced renal fibrosis
|
| Treatment protocol |
Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
|
| Growth protocol |
All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
RMA by software Partek
|
| |
|
| Submission date |
Jan 27, 2014 |
| Last update date |
Aug 08, 2018 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE54441 |
Differences among inbred mouse strains in sensitivity to TGF-ß1 induced renal fibrosis: association with TIMP-1 and EGR2 expression |
|
