The isolation of total RNA from whole blood was performed using the ZR Whole-Blood Total RNA Kit™ (Zymo Research Corp., Irvine, CA, USA) according to the manufacturer’s recommendations.
Label
biotin
Label protocol
Standard Illumina protocol
Hybridization protocol
Standard Illumina protocol
Scan protocol
Standard Illumina protocol
Data processing
The software package Genome Studio (Illumina, San Diego, California, USA) and the DAVID Bioinformatics Resources 6.7 database (http://david.abcc.ncifcrf.gov/home.jsp) were used for the statistical processing. Normalized signal intensity was used for further investigation. A volcano plot was used to identify differentially expressed genes (using n-fold > 1.5 and P < 0.01 as the threshold of statistical significance). For further investigations, we selected transcripts that were common among all the pairs and pools.
