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Sample GSM1322065 Query DataSets for GSM1322065
Status Public on Nov 03, 2014
Title tamoxifen-day 2-1
Sample type RNA
 
Source name mouse myocardium, left ventricle
Organism Mus musculus
Characteristics strain: C57Bl6/J
gender: male
age: 12-20 weeks
tissue: myocardium, left ventricle
Treatment protocol For intraperitoneal injection, 10mg tamoxifen free base (T5648, Sigma) was dissolved in 1mL peanut oil (Sigma) and then sonicated. 100microL (1.0 mg tamoxifen) was injected daily (40mg/kg/day, i.p.) for 5 days.
Growth protocol All mice were supplied with the same diet and water ad libitum; Mice were maintained on a 12-hour light/dark cycle in a temperature and humidity controlled environment.
Extracted molecule total RNA
Extraction protocol Total RNA was preparted using TRIzol reagent (Invitrogen) from left ventricular myocardial tissue. Total RNA yield and purity (260nm:280nm) were determined spectrophotometrically. The integrity (RIN>9.0) of the re-suspended total RNA was determined using the RNA Nano Chip Kit on the Bioanalyzer 2100 and the 2100 Expert software (Agilent).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description Tamoxifen x2 days -1
Data processing The data were normalized using quantile normalization with Solo, a simple standalone tood implementing the quantile-quantile normalization method (Univ. of Strasbourg, France)
 
Submission date Feb 05, 2014
Last update date Nov 03, 2014
Contact name Andreas Sebastian Barth
E-mail(s) abarth3@jhmi.edu
Phone +1 410 614 0032
Organization name Johns Hopkins University
Department Medicine, Division of Cardiology
Lab Tomaselli
Street address 720 Rutland Ave.
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
 
Platform ID GPL6885
Series (1)
GSE54681 Genome-wide changes in gene expression during the development and after recovery from heart failure in a mouse model of transient cardiomyopathy.

Data table header descriptions
ID_REF
VALUE Quantile normalization.
JR1-2.Detection Pval

Data table
ID_REF VALUE JR1-2.Detection Pval
ILMN_2896528 5797.43 0
ILMN_2721178 1063.35 0
ILMN_3033922 1203.89 0
ILMN_3092673 4163.25 0
ILMN_2816356 237.347 0.01128
ILMN_2808939 349.958 0
ILMN_2634564 466.337 0
ILMN_2737647 178.595 0.19549
ILMN_2734484 1076.62 0
ILMN_2952292 185.584 0.12907
ILMN_2699078 159.958 0.55263
ILMN_1213681 1342.18 0
ILMN_2735413 170 0.32206
ILMN_2735415 183.963 0.14286
ILMN_2891688 1633.58 0
ILMN_2637698 1667.57 0
ILMN_2674228 404.453 0
ILMN_2601546 207.068 0.0401
ILMN_1230831 153.379 0.69424
ILMN_2848071 169.047 0.33584

Total number of rows: 25697

Table truncated, full table size 673 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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