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Sample GSM1322074 Query DataSets for GSM1322074
Status Public on Nov 03, 2014
Title tamoxifen-day 10-3
Sample type RNA
 
Source name mouse myocardium, left ventricle
Organism Mus musculus
Characteristics strain: C57Bl6/J
gender: male
age: 12-20 weeks
tissue: myocardium, left ventricle
Treatment protocol For intraperitoneal injection, 10mg tamoxifen free base (T5648, Sigma) was dissolved in 1mL peanut oil (Sigma) and then sonicated. 100microL (1.0 mg tamoxifen) was injected daily (40mg/kg/day, i.p.) for 5 days.
Growth protocol All mice were supplied with the same diet and water ad libitum; Mice were maintained on a 12-hour light/dark cycle in a temperature and humidity controlled environment.
Extracted molecule total RNA
Extraction protocol Total RNA was preparted using TRIzol reagent (Invitrogen) from left ventricular myocardial tissue. Total RNA yield and purity (260nm:280nm) were determined spectrophotometrically. The integrity (RIN>9.0) of the re-suspended total RNA was determined using the RNA Nano Chip Kit on the Bioanalyzer 2100 and the 2100 Expert software (Agilent).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description Tamoxifen x5 days + 5 days normal diet -3
Data processing The data were normalized using quantile normalization with Solo, a simple standalone tood implementing the quantile-quantile normalization method (Univ. of Strasbourg, France)
 
Submission date Feb 05, 2014
Last update date Nov 03, 2014
Contact name Andreas Sebastian Barth
E-mail(s) abarth3@jhmi.edu
Phone +1 410 614 0032
Organization name Johns Hopkins University
Department Medicine, Division of Cardiology
Lab Tomaselli
Street address 720 Rutland Ave.
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
 
Platform ID GPL6885
Series (1)
GSE54681 Genome-wide changes in gene expression during the development and after recovery from heart failure in a mouse model of transient cardiomyopathy.

Data table header descriptions
ID_REF
VALUE Quantile normalization.
R120-4.Detection Pval

Data table
ID_REF VALUE R120-4.Detection Pval
ILMN_2896528 4538.62 0
ILMN_2721178 780.321 0
ILMN_3033922 1556.44 0
ILMN_3092673 4308.16 0
ILMN_2816356 214.779 0.00877
ILMN_2808939 588.253 0
ILMN_2634564 586.311 0
ILMN_2737647 168.121 0.3584
ILMN_2734484 1047.27 0
ILMN_2952292 221.589 0.00376
ILMN_2699078 141.853 0.91479
ILMN_1213681 554.584 0
ILMN_2735413 153.305 0.71303
ILMN_2735415 162.695 0.48371
ILMN_2891688 1281.33 0
ILMN_2637698 1463.65 0
ILMN_2674228 556.4 0
ILMN_2601546 190.053 0.08897
ILMN_1230831 155.679 0.65288
ILMN_2848071 230.416 0.00376

Total number of rows: 25697

Table truncated, full table size 676 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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