|
| Status |
Public on Nov 03, 2014 |
| Title |
tamoxifen-day 28-3 |
| Sample type |
RNA |
| |
|
| Source name |
mouse myocardium, left ventricle
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6/J
gender: male
age: 12-20 weeks
tissue: myocardium, left ventricle
|
| Treatment protocol |
For intraperitoneal injection, 10mg tamoxifen free base (T5648, Sigma) was dissolved in 1mL peanut oil (Sigma) and then sonicated. 100microL (1.0 mg tamoxifen) was injected daily (40mg/kg/day, i.p.) for 5 days.
|
| Growth protocol |
All mice were supplied with the same diet and water ad libitum; Mice were maintained on a 12-hour light/dark cycle in a temperature and humidity controlled environment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was preparted using TRIzol reagent (Invitrogen) from left ventricular myocardial tissue. Total RNA yield and purity (260nm:280nm) were determined spectrophotometrically. The integrity (RIN>9.0) of the re-suspended total RNA was determined using the RNA Nano Chip Kit on the Bioanalyzer 2100 and the 2100 Expert software (Agilent).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Tamoxifen x5 days + 23 days normal diet -3
|
| Data processing |
The data were normalized using quantile normalization with Solo, a simple standalone tood implementing the quantile-quantile normalization method (Univ. of Strasbourg, France)
|
| |
|
| Submission date |
Feb 05, 2014 |
| Last update date |
Nov 03, 2014 |
| Contact name |
Andreas Sebastian Barth |
| E-mail(s) |
abarth3@jhmi.edu
|
| Phone |
+1 410 614 0032
|
| Organization name |
Johns Hopkins University
|
| Department |
Medicine, Division of Cardiology
|
| Lab |
Tomaselli
|
| Street address |
720 Rutland Ave.
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE54681 |
Genome-wide changes in gene expression during the development and after recovery from heart failure in a mouse model of transient cardiomyopathy. |
|
