|
| Status |
Public on Dec 31, 2014 |
| Title |
Gene expression in HMEC |
| Sample type |
RNA |
| |
|
| Source name |
Normal mammary epithelial cells, HMEC
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HMEC
|
| Growth protocol |
Du145, PC3, MCF7, and MDA-MB-231 were maintained in RPMI1640 medium. RWPE1 was maintained in keratinocyte-SFM. HMEC was maintained in mammary epithelial cell serum-free growth medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cancer cell lines and normal cells using ISOGEN (Nippon Gene, Tokyo, Japan).
|
| Label |
Cy3
|
| Label protocol |
From 200 ng of total RNA, Cy3-labeled cRNA was synthesized using Low Input Quick Amp Labeling Kit (Agilent technologies).
|
| |
|
| Hybridization protocol |
600 ng of labeled cRNA was hybridized to SurePrint G3 Human GE v2 8x60K Microarray for 17 hours at 65℃ and 10 rpm rotation. Microarrays was washed according to Agilent protocol.
|
| Scan protocol |
The microarray was scanned with an Agilent G2565BA microarray scanner (Agilent Technologies).
|
| Description |
Untreated cells
|
| Data processing |
Scanned data was analyzed with Feature Extraction Ver.9.1 and analyzed using GeneSpring Ver.12.5 software (Agilent Technologies).
|
| |
|
| Submission date |
Feb 06, 2014 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Hideyuki Takeshima |
| E-mail(s) |
takeshima.hideyuki@hoshi.ac.jp
|
| Organization name |
Hoshi University
|
| Department |
Institute for Advanced Life Sciences
|
| Lab |
Department of Epigenomics
|
| Street address |
2-4-41 Ebara
|
| City |
Shinagawa-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
142-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE54758 |
DNA methylation, H3K27me3, and gene expression statuses of human cancer cell lines and normal cells. |
|
