|
| Status |
Public on Mar 06, 2014 |
| Title |
841SPL Tg |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Spleen
disease: Clinically Healthy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Genomic DNA was extracted using standard techniques
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
DNA was bisulfite converted, amplified, and labeled
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
DNA was hybridized to the Illumina GoldenGate Cancer Panel I array using the standard Illumina protocol
|
| Scan protocol |
Standard Illumina scanning protocol
Arrays were imaged using BeadArray Reader using the standard Illumina scanner setting
|
| Description |
Clinically Healthy
|
| Data processing |
Intensity was generated by the Illumina BeadStudio software and processed by the R/Bioconductor packages
Cy3, Cy5, Average Beta, and detection p-values were extracted using BeadStudio software
|
| |
|
| Submission date |
Feb 07, 2014 |
| Last update date |
Mar 06, 2014 |
| Contact name |
Yuelin Jack Zhu |
| E-mail(s) |
zhujack@mail.nih.gov
|
| Phone |
(240)760-7439
|
| Organization name |
NCI
|
| Department |
Genetics Branch, Center for Cancer Research
|
| Lab |
Dr. Paul Meltzer's Lab
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE54787 |
Gene expression study of leukemic and premalignant hematopoietic tissues from NUP98-PHF23 (NP23) mice. |
| GSE54788 |
NUP98-PHF23 |
|
