|
| Status |
Public on Mar 01, 2014 |
| Title |
FcgKO IFNg stim 2 |
| Sample type |
RNA |
| |
|
| Source name |
FcgKO IFNg stim 2
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: Fcg-chain KO
cell type: Peritoneal MF
|
| Treatment protocol |
Peritoneal MF were stimulated with recombinant IFNg (10 ng/ml) for 6hrs.
|
| Growth protocol |
Peritoneal MF were isolated from naïve euthanized mouse and plated in 24-well TC dishes into complete RPMI media, with 10% FBS and 15% L929 conditional sup containing MCSF o/n to allow MF adherence. Floaters (B cells, T cells etc) were discarded, fresh pre-warmed media was added over MF and cells stimulated with IFNg for 6hrs before RNA collection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from stimulated or control peritoneal MF was isolated using Qiagen RNEasy Kit. DNA was removed using Qiagen Dnase protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the Ambion Illumina® TotalPrepTM RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol with Cy3-SAV
|
| Scan protocol |
Standard Illumina scanning protocol using HiScan System
|
| Description |
N/A
|
| Data processing |
The data were analysed in Genome Studio Illumina Software
|
| |
|
| Submission date |
Feb 10, 2014 |
| Last update date |
Mar 01, 2014 |
| Contact name |
Ruslan Medzhitov |
| Organization name |
Yale University
|
| Department |
Immunobiology
|
| Street address |
PO Box 208011 300 Cedar Street TAC S660
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE54812 |
WT and FcgKO peritoneal MF response to IFNg |
| GSE54824 |
A role for the ITAM signaling module in specifying cytokine receptor functions |
|
