|
| Status |
Public on Feb 10, 2014 |
| Title |
MEB55 6hr_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MEB55 6hr, U2OS cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U2OS
agent: MEB55
time: 6hr
|
| Treatment protocol |
Cells were treated with 5 ppm of either ST362 or MEB55 (both analogues of strigolactones) dissolved in acetone for either 6 hr or 24 hr. control cells were treated with vehicle alone with the amount corresponding to treatment groups.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from cells with Trizol reagent according to manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the Illumina TotalPrep-96 RNA Amplification Kit (By Ambion, Inc.) starting with 200ng total RNA- according to the manufacturer’s directions.
|
| |
|
| Hybridization protocol |
Hybridization and washing of HumanHT-12_v4_BeadChip was performed according to the Whole-Genome Gene Expression Direct Hybridization Assay Guide (Illumina, Catalog # BD-901-1002, Part # 11322355 Rev. A).
|
| Scan protocol |
Twelve samples were run on one BeadChip and Illumina iScan Control Software was used for scanning.
|
| Description |
SAMPLE 5
U2OS cells are osteosarcoma cell line originated from a bone tissue and available from ATCC (originated from 15 yrs old caucasian female).
|
| Data processing |
Data were extracted using GenomeStudio software. Background was subtracted and the data were normalized using quantile function.
|
| |
|
| Submission date |
Feb 10, 2014 |
| Last update date |
Feb 10, 2014 |
| Contact name |
Ronit Iris Yarden |
| E-mail(s) |
riy2@georgetown.edu
|
| Phone |
202-6876872
|
| Organization name |
Georgetown University
|
| Department |
Human Science
|
| Street address |
3900 Reservoir Rd., NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20007 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE54820 |
Strigolactone analogs induce apoptosis through activation of p38 and the stress response pathway in cancer cell lines and in conditionally reprogrammed primary prostate cancer cells |
|
