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Sample GSM1324491 Query DataSets for GSM1324491
Status Public on Mar 01, 2014
Title FcgKO IL4 stim 1
Sample type RNA
 
Source name Peritoneal MF
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: Fcg-chain KO
cell type: Peritoneal MF
Treatment protocol Peritoneal MF were stimulated with recombinant IL4 (10 ng/ml) for 6hrs.
Growth protocol Peritoneal MF were isolated from naïve euthanized mouse and plated in 24-well TC dishes into complete RPMI media, with 10% FBS and 15% L929 conditional sup containing MCSF o/n to allow MF adherence. Floaters (B cells, T cells etc) were discarded, fresh pre-warmed media was added over MF and cells stimulated with IL4 for 6hrs before RNA collection
Extracted molecule total RNA
Extraction protocol Total RNA from stimulated or control peritoneal MF was isolated using Qiagen RNEasy Kit. DNA was removed using Qiagen Dnase protocol.
Label biotin
Label protocol Biotinylated cRNA was prepared using the Ambion Illumina® TotalPrepTM RNA Amplification Kit
 
Hybridization protocol Standard Illumina hybridization protocol with Cy3-SAV
Scan protocol Standard Illumina scanning protocol using HiScan System
Description N/A
Data processing The data were analysed in Genome Studio Illumina Software
 
Submission date Feb 10, 2014
Last update date Mar 01, 2014
Contact name Ruslan Medzhitov
Organization name Yale University
Department Immunobiology
Street address PO Box 208011 300 Cedar Street TAC S660
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platform ID GPL6885
Series (2)
GSE54823 WT and FcgKO peritoneal MF response to IL4
GSE54824 A role for the ITAM signaling module in specifying cytokine receptor functions

Data table header descriptions
ID_REF
VALUE Quantile normalized values obtained from the Genome Studio Software analysis
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 533.1041 0
ILMN_2721178 237.8586 0
ILMN_3033922 502.1617 0
ILMN_3092673 1620.748 0
ILMN_2816356 104.5704 0.003759399
ILMN_2808939 338.9381 0
ILMN_2634564 283.784 0
ILMN_2737647 91.73097 0.2756892
ILMN_2734484 157.4468 0
ILMN_2952292 134.5823 0
ILMN_2699078 87.53067 0.7205514
ILMN_1213681 152.5756 0
ILMN_2735413 89.74059 0.4561403
ILMN_2735415 91.98262 0.2568922
ILMN_2891688 308.1908 0
ILMN_2637698 707.2827 0
ILMN_2674228 260.0246 0
ILMN_2601546 92.36605 0.2243108
ILMN_1230831 99.77223 0.03383459
ILMN_2848071 102.7045 0.0112782

Total number of rows: 25697

Table truncated, full table size 733 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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