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Sample GSM1324493 Query DataSets for GSM1324493
Status Public on Mar 01, 2014
Title FcgKO Unstimulated 2
Sample type RNA
Source name Peritoneal MF
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: Fcg-chain KO
cell type: Peritoneal MF
Growth protocol Peritoneal MF were isolated from naïve euthanized mouse and plated in 24-well TC dishes into complete RPMI media, with 10% FBS and 15% L929 conditional sup containing MCSF o/n to allow MF adherence. Floaters (B cells, T cells etc) were discarded, fresh pre-warmed media was added over MF and cells stimulated with IL4 for 6hrs before RNA collection
Extracted molecule total RNA
Extraction protocol Total RNA from stimulated or control peritoneal MF was isolated using Qiagen RNEasy Kit. DNA was removed using Qiagen Dnase protocol.
Label biotin
Label protocol Biotinylated cRNA was prepared using the Ambion Illumina® TotalPrepTM RNA Amplification Kit
Hybridization protocol Standard Illumina hybridization protocol with Cy3-SAV
Scan protocol Standard Illumina scanning protocol using HiScan System
Description N/A
Data processing The data were analysed in Genome Studio Illumina Software
Submission date Feb 10, 2014
Last update date Mar 01, 2014
Contact name Ruslan Medzhitov
Organization name Yale University
Department Immunobiology
Street address PO Box 208011 300 Cedar Street TAC S660
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
Platform ID GPL6885
Series (2)
GSE54823 WT and FcgKO peritoneal MF response to IL4
GSE54824 A role for the ITAM signaling module in specifying cytokine receptor functions

Data table header descriptions
VALUE Quantile normalized values obtained from the Genome Studio Software analysis
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 558.0184 0
ILMN_2721178 220.1273 0
ILMN_3033922 478.799 0
ILMN_3092673 1741.417 0
ILMN_2816356 97.45193 0.03884712
ILMN_2808939 419.603 0
ILMN_2634564 244.1453 0
ILMN_2737647 91.01052 0.3120301
ILMN_2734484 161.9895 0
ILMN_2952292 131.4332 0
ILMN_2699078 89.63481 0.4423559
ILMN_1213681 152.0366 0
ILMN_2735413 91.0619 0.3057644
ILMN_2735415 89.37738 0.4686717
ILMN_2891688 277.1516 0
ILMN_2637698 640.7832 0
ILMN_2674228 220.2181 0
ILMN_2601546 91.92816 0.2343358
ILMN_1230831 97.21093 0.04010025
ILMN_2848071 97.47247 0.03884712

Total number of rows: 25697

Table truncated, full table size 728 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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