|
| Status |
Public on Apr 01, 2014 |
| Title |
coc-14_A |
| Sample type |
RNA |
| |
|
| Source name |
coc-14
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: cocaine addiction
organ: Brain
tissue: Ventral midbrain
sample type: Postmortem
Cause of death: cocaine related
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalized using quantile normalization with Illumina BeadStudio. They were also batch normalized.
|
| |
|
| Submission date |
Feb 10, 2014 |
| Last update date |
Apr 01, 2014 |
| Contact name |
Michael J Bannon |
| E-mail(s) |
mbannon@med.wayne.edu
|
| Phone |
(313) 993-4271
|
| Organization name |
Wayne State University School of Medicine
|
| Department |
Pharmacology
|
| Lab |
3302 Scott Hall
|
| Street address |
540 E Canfield
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48202 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE54839 |
A molecular profile of chronic cocaine abuse includes differential expression of genes regulating transcription, chromatin and dopamine cell phenotype |
|
