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Sample GSM1328038 Query DataSets for GSM1328038
Status Public on Feb 20, 2014
Title Cell 1
Sample type RNA
 
Source name Cell Normoxia Pair 1
Organism Homo sapiens
Characteristics cell line: Molm-14
oxygen condition: normoxia
sample group: Parental cells
sample pair id: pair 1
Treatment protocol Cells were incubated in BioSpherix hypoxia chamber under hypoxic conditions (1% O2) or in a Thermo Scientific cell culture incubator under “normoxic” conditions (20% O2), in all cases at 37C and 5% CO2.
Growth protocol Cells were cultured in RPMI + 1X Pen/Strep, 10% exosome-free FBS (prepared through ultracentrifugation of FBS - 100,000xg for 6h).
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Qiagen miRNEasy kit
Label biotin
Label protocol 130ng RNA was labeled using the Affymetrix HSR miRNA Flash Tag Biotin kit
 
Hybridization protocol 130ng labeled RNA was hybridized for 18 hr at 48C on GeneChip miRNA 3.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description Parental cell 1 under normoxia
CellNO
Data processing The data were analyzed with Partek Genomcis Suite(v.6.6) using the RMA normalization method
 
Submission date Feb 13, 2014
Last update date Feb 20, 2014
Contact name Amiee Bell Potter
E-mail(s) potteram@ohsu.edu
Phone 503-418-9376
Organization name OHSU
Department Integrated Genomics Laboratory
Lab GPSR
Street address 3181 SW Sam Jackson Park Rd, Mail Code #L581
City Portland
State/province OR
ZIP/Postal code 97239
Country USA
 
Platform ID GPL16384
Series (1)
GSE55025 Expression and incorporation of microRNA in exosomes released by leukemia cells under room air and hypoxia

Data table header descriptions
ID_REF
VALUE RMA Log2 Transformed Signal Intensity

Data table
ID_REF VALUE
14q0_st 0.884745
14qI-1_st 1.34433
14qI-1_x_st 1.16146
14qI-2_st 1.05792
14qI-3_x_st 1.03684
14qI-4_st 0.972326
14qI-4_x_st 0.8323
14qI-5_st 1.16787
14qI-6_st 1.08124
14qI-6_x_st 0.879433
14qI-7_st 1.03485
14qI-8_st 1.1686
14qI-8_x_st 1.23856
14qI-9_x_st 1.26215
14qII-10_st 0.410294
14qII-10_x_st 1.48264
14qII-11_st 0.843303
14qII-11_x_st 0.890171
14qII-12_st 1.0774
14qII-12_x_st 0.962614

Total number of rows: 25119

Table truncated, full table size 622 Kbytes.




Supplementary file Size Download File type/resource
GSM1328038_002A_H1_Cell_564PK.CEL.gz 758.1 Kb (ftp)(http) CEL
Processed data included within Sample table

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