|
| Status |
Public on Feb 20, 2014 |
| Title |
Microvesicle Hypoxia 1 |
| Sample type |
RNA |
| |
|
| Source name |
Exosome Hypoxia Pair 4
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Molm-14
oxygen condition: hypoxia
sample group: Released exosome
sample pair id: pair 4
|
| Treatment protocol |
Cells were incubated in BioSpherix hypoxia chamber under hypoxic conditions (1% O2) or in a Thermo Scientific cell culture incubator under “normoxic” conditions (20% O2), in all cases at 37C and 5% CO2.
|
| Growth protocol |
Cells were cultured in RPMI + 1X Pen/Strep, 10% exosome-free FBS (prepared through ultracentrifugation of FBS - 100,000xg for 6h).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen miRNEasy kit
|
| Label |
biotin
|
| Label protocol |
130ng RNA was labeled using the Affymetrix HSR miRNA Flash Tag Biotin kit
|
| |
|
| Hybridization protocol |
130ng labeled RNA was hybridized for 18 hr at 48C on GeneChip miRNA 3.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Released exosome 1 under hypoxia
MVHO
|
| Data processing |
The data were analyzed with Partek Genomcis Suite(v.6.6) using the RMA normalization method
|
| |
|
| Submission date |
Feb 13, 2014 |
| Last update date |
Feb 20, 2014 |
| Contact name |
Amiee Bell Potter |
| E-mail(s) |
potteram@ohsu.edu
|
| Phone |
503-418-9376
|
| Organization name |
OHSU
|
| Department |
Integrated Genomics Laboratory
|
| Lab |
GPSR
|
| Street address |
3181 SW Sam Jackson Park Rd, Mail Code #L581
|
| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97239 |
| Country |
USA |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE55025 |
Expression and incorporation of microRNA in exosomes released by leukemia cells under room air and hypoxia |
|
