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Sample GSM1329050 Query DataSets for GSM1329050
Status Public on Feb 18, 2014
Title WT
Sample type RNA
 
Source name CD11c+CD80+CD86+MHC-II+
Organism Mus musculus
Characteristics treatment: PBS
genotype: wild type
genetic background: C56BL/6
cell type: Spleen dendritic cells
Treatment protocol Pure dendritic cells from the spleens of WT and MARCO KO mice were cultured overnight in PBS or Lipopolysaccharide (LPS).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol.
Label Biotin
Label protocol Biotin-UTP was incorporated during the overnight in vitro transcription step according to the manufacturer's (Ambion® | Life Technologies) protocol.
 
Hybridization protocol cRNA (15 μg) was fragmented and hybridized to arrays according to the manufacturer's (Ambion® | Life Technologies) protocols.
Scan protocol Arrays were scanned with the GeneChip Scanner 3000 and GeneChip Operating Software acquired raw fluorescence signal.
Description WT DC + Buffer
Data processing Scanned array images and CEL file data were analyzed and normalized using RMAexpress.
 
Submission date Feb 15, 2014
Last update date Feb 18, 2014
Contact name M. Simo Arredouani
E-mail(s) simarred@gmail.com
Organization name Beth Israel Deaconess Medical Center
Department Surgery
Street address 330 Brookline Ave.
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL11180
Series (1)
GSE55068 Contribution of the scavenger receptor MARCO to TLR-induced activation of dendritic cells

Data table header descriptions
ID_REF
VALUE Linear intensity output from RMAexpress

Data table
ID_REF VALUE
1415670_PM_at 90.76542
1415671_PM_at 187.10472
1415672_PM_at 519.2406
1415673_PM_at 47.30967
1415674_PM_a_at 119.112755
1415675_PM_at 54.845062
1415676_PM_a_at 138.34506
1415677_PM_at 80.93078
1415678_PM_at 89.1022
1415679_PM_at 348.3721
1415680_PM_at 101.39774
1415681_PM_at 175.30481
1415682_PM_at 37.15611
1415683_PM_at 435.43652
1415684_PM_at 47.06987
1415685_PM_at 61.739574
1415686_PM_at 94.32635
1415687_PM_a_at 1989.3108
1415688_PM_at 205.6022
1415689_PM_s_at 100.002205

Total number of rows: 45141

Table truncated, full table size 1040 Kbytes.




Supplementary file Size Download File type/resource
GSM1329050_WT.CEL.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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