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Sample GSM1329053 Query DataSets for GSM1329053
Status Public on Feb 18, 2014
Title MARCO_LPS
Sample type RNA
 
Source name CD11c+CD80+CD86+MHC-II+
Organism Mus musculus
Characteristics treatment: LPS
genotype: MARCO-/-
genetic background: C56BL/6
cell type: Spleen dendritic cells
Treatment protocol Pure dendritic cells from the spleens of WT and MARCO KO mice were cultured overnight in PBS or Lipopolysaccharide (LPS).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol.
Label Biotin
Label protocol Biotin-UTP was incorporated during the overnight in vitro transcription step according to the manufacturer's (Ambion® | Life Technologies) protocol.
 
Hybridization protocol cRNA (15 μg) was fragmented and hybridized to arrays according to the manufacturer's (Ambion® | Life Technologies) protocols.
Scan protocol Arrays were scanned with the GeneChip Scanner 3000 and GeneChip Operating Software acquired raw fluorescence signal.
Description MARCO DC + LPS
Data processing Scanned array images and CEL file data were analyzed and normalized using RMAexpress.
 
Submission date Feb 15, 2014
Last update date Feb 18, 2014
Contact name M. Simo Arredouani
E-mail(s) simarred@gmail.com
Organization name Beth Israel Deaconess Medical Center
Department Surgery
Street address 330 Brookline Ave.
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL11180
Series (1)
GSE55068 Contribution of the scavenger receptor MARCO to TLR-induced activation of dendritic cells

Data table header descriptions
ID_REF
VALUE Linear intensity output from RMAexpress

Data table
ID_REF VALUE
1415670_PM_at 135.49608
1415671_PM_at 232.35526
1415672_PM_at 423.22964
1415673_PM_at 120.30285
1415674_PM_a_at 193.57364
1415675_PM_at 77.67364
1415676_PM_a_at 354.5145
1415677_PM_at 52.171642
1415678_PM_at 88.76751
1415679_PM_at 420.5115
1415680_PM_at 93.73878
1415681_PM_at 201.23367
1415682_PM_at 47.896202
1415683_PM_at 677.0593
1415684_PM_at 56.22377
1415685_PM_at 73.07363
1415686_PM_at 87.59209
1415687_PM_a_at 641.3624
1415688_PM_at 249.04297
1415689_PM_s_at 126.82896

Total number of rows: 45141

Table truncated, full table size 1040 Kbytes.




Supplementary file Size Download File type/resource
GSM1329053_MARCO_LPS.CEL.CEL.gz 1.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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