|
| Status |
Public on Sep 15, 2006 |
| Title |
SKI-DLCL_tumor_animals treated with 1mg/kg Aplidin_50mg/kg AraC |
| Sample type |
RNA |
| |
|
| Source name |
RNA isolated from SKI-DLCL tumor harvested from mice treated with Aplidin and AraC
|
| Organism |
Homo sapiens |
| Characteristics |
SKI-DLCL tumor grown in CB-17 scid/scid mice. Tumor was harvested on day eight following the treatment. Total RNA was extracted using RNeasy kit.
|
| Biomaterial provider |
own
|
| Treatment protocol |
Mice were given a single dose of 1mg/kg Aplidin and 50mg/kg AraC.
|
| Growth protocol |
Mice were kept in CINJ vivarium under standard condition. SKI-DLCL cells were injected s.c.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy kit from Qiagen
|
| Label |
biotin
|
| Label protocol |
Synthesis of Biotin-Labeled cRNA/Purification
ENZO BioArray RNA Transcript Labeling Kit
GeneChip Sample Cleanup Module
Miscellaneous Reagents/Supplies
100% ethanol room temperature
80% ethanol room temperature
Water, Molecular Biology Grade or DEPC-treated
Ethidium Bromide
Sterile, RNase-free, microcentrifuge tubes, 1.5mL
Micropipettors
Sterile-barrier, RNase-free pipette tips
Mini agarose gel electrophoresis unit with appropriate buffers
UV Spectrophotometer
Cooling water bath
|
| |
|
| Hybridization protocol |
Hybridization
Water, Molecular Biology Grade or DEPC-treated
Acetylated Bovine Serum Albumin (BSA) solution (50 mg/mL)
Herring Sperm DNA
GeneChip Eukaryotic Hybridization Control Kit
5M NaCl, RNase-free, DNase-free
MES Free Acid Monohydrate
MES Sodium Salt
EDTA Disodium Salt
Miscellaneous Reagents/Supplies
Surfact-Amps 20 (Tween-20), 10%
Hybridization Oven 640
Sterile, RNase-free, microcentrifuge tubes, 1.5mL
Micropipettors
Sterile-barrier pipette tips and non-barrier tips
Heatblock
|
| Scan protocol |
Washing, Staining, and Scanning
Water, Molecular Biology Grade
Distilled water
Acetylated Bovine Serum Albumin (BSA) solution (50 mg/mL)
R-Phycoerythrin Streptavidin
20X SSPE (3M NaCl, 0.2M NaH2PO4, 0.02M EDTA)
Goat IgG, Reagent Grade
Anti-streptavidin antibody (goat), biotinylated
10% surfact-Amps 20 (Tween-20)
|
| Description |
Sample processed by CINJ core expression array facility according to Affymetrix protocol.
|
| Data processing |
scanned fluorescence intensity values, not normalized
|
| |
|
| Submission date |
Aug 31, 2006 |
| Last update date |
Dec 23, 2010 |
| Contact name |
Pravin Mishra |
| E-mail(s) |
mishrapr@umdnj.edu
|
| Organization name |
UMDNJ
|
| Department |
Pharmacology
|
| Lab |
Bertino/Banerjee
|
| Street address |
195 Little Albany St.
|
| City |
New Brunswick |
| State/province |
NJ |
| ZIP/Postal code |
08904 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE5681 |
Aplidin synergizes with cytosine arabinoside: functional relevance of mitochondria in Aplidin-induced cytotoxicity |
|
