|
Status |
Public on Jun 01, 2015 |
Title |
liver_wt_rep1 [BH4 study] |
Sample type |
RNA |
|
|
Source name |
liver
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J genotype/variation: wild type allels: Pts-R15C-ki age: 17 weeks mouse id: 30164244 gender: male tissue: liver
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol using HiScan
|
Description |
SAMPLE 9 replicate 1
|
Data processing |
The data were normalized using cubic spline normalization with Illumina Genomestudio 2011.1
|
|
|
Submission date |
Feb 19, 2014 |
Last update date |
Jun 01, 2015 |
Contact name |
Martin Irmler |
Organization name |
Helmholtz Zentrum München GmbH
|
Department |
Institute of Experimental Genetics
|
Lab |
Gene Regulation & Epigenetics
|
Street address |
Ingolstaedter Landstrasse 1
|
City |
Neuherberg |
State/province |
Bayern |
ZIP/Postal code |
85764 |
Country |
Germany |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE55148 |
Mildly compromised tetrahydrobiopterin biosynthesis mouse mutants exhibit abnormal body fat distribution and abdominal obesity |
|