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Sample GSM1330621 Query DataSets for GSM1330621
Status Public on Feb 20, 2014
Title Old5_Mouse430
Sample type RNA
 
Source name distal trachea and carina region of 14 month old mice
Organism Mus musculus
Characteristics background strain: C57Bl/6
gender: female
age: 14 month
tissue: distal trachea and carina region
Treatment protocol Distal tracheas and carinas of four young (2 month) and four older (14 month) C57Bl/6 female mice were dissected out.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the distal tracheas and carinas using QIAshredder and RNeasy Micro Kits (QIAGEN).
Label Biotin
Label protocol Total RNA was processed using Ambion MessageAmpTM Premier by the Duke Microarray Facility.
 
Hybridization protocol Affymetrix ENZO labeling kit
Scan protocol Hybridization of samples to Affymetrix GeneChip arrays was performed according to Affymetrix manual instructions
Data processing Scanning of Affymetrix GeneChip arrays was performed according to the Affmetrix manual instructions. Data analysis was performed using Genomics Suite 6.5 (Partek) software and robust multichip analysis normalization was performed on each dataset. Two-way ANOVA and fold-change analyses were performed to select genes that were differentially expressed between 2 month and 14 month old C57Bl/6 datasets. Top differentially expressed genes were selected with a p-value cutoff of <0.05 based on ANOVA test and a fold-change cutoff of >±2.0.
 
Submission date Feb 19, 2014
Last update date Feb 20, 2014
Contact name Brigid Hogan
E-mail(s) brigid.hogan@duke.edu
Phone 919-684-8085
Organization name Duke University Medical Center
Department Cell Biology
Lab Hogan Lab
Street address 388 Nanaline Duke Bldg PO Box 3709
City Durham
State/province North Carolina
ZIP/Postal code 27710
Country USA
 
Platform ID GPL1261
Series (1)
GSE55162 Age-related changes in the cellular composition and epithelial organization of the mouse trachea

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
1415670_at 1674.207 P
1415671_at 3800.744 P
1415672_at 3991.513 P
1415673_at 536.3834 P
1415674_a_at 1347.591 P
1415675_at 974.0235 P
1415676_a_at 2779.071 P
1415677_at 410.2812 P
1415678_at 2413.927 P
1415679_at 4199.124 P
1415680_at 595.9946 P
1415681_at 1379.6 P
1415682_at 563.3948 P
1415683_at 2187.59 P
1415684_at 686.939 P
1415685_at 808.4703 P
1415686_at 1763.911 P
1415687_a_at 8267.587 P
1415688_at 2725.401 P
1415689_s_at 1226.647 P

Total number of rows: 45101

Table truncated, full table size 982 Kbytes.




Supplementary file Size Download File type/resource
GSM1330621_3106_10769_37726_Old5_Mouse430+2.CEL.gz 3.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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