The three different CO2 concentration treatments were: T0 treatment (385 ppm, day 25˚C/night 20˚C), T1 treatment (550 ppm, day 28˚C/night 23˚C) and T2 treatment (720 ppm, day 31˚C/night 26˚C).
Growth protocol
Homogeneous 20 cm long, woody-stem cuttings of triploid white poplar were planted in 20 cm*26 cm*34 cm plastic pots. After growth for about three months, twenty randomly selected pots were moved into three controlled environment chambers (Beijing, China).Ten days later, they were subjected to different CO2 concentrations treatments three months.
Extracted molecule
total RNA
Extraction protocol
total RNA
Label
biotin
Label protocol
biotin
Hybridization protocol
Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
Scan protocol
Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
Description
Gene expression data from leaves under normal CO2 concentration
Data processing
Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
