|
| Status |
Public on Jul 02, 2014 |
| Title |
6 Etoh 2 |
| Sample type |
RNA |
| |
|
| Source name |
Alveolar type 2 cells_Alcohol ingestion
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain background: Sprague Dawley
gender: male
diet: Lieber-DeCarlie diet containg EtOH for 6 wk
sample group: Alcohol ingestion
cell type: Alveolar type 2 cells
|
| Treatment protocol |
Alveolar type 2 cells were isolated using an immunoabsorption technique.
|
| Growth protocol |
Male Spargue Dawley rats were fed the liquid Lieber-DeCarlie diet containg EtOH for 6 wk or an isocaloric control diet without EtOH.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by Trizol.
|
| Label |
biotin
|
| Label protocol |
Biotinylation of RNA was completed as described in the Affymetrix user manual.
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol
|
| Scan protocol |
Mouse Genome 430 2.0 GeneChips were scanned using the Affymetrix 3000 scanner/AGCC.
|
| Description |
6 Etoh 2.CEL
|
| Data processing |
Data were analyzed by GeneSpring GX.
|
| |
|
| Submission date |
Feb 21, 2014 |
| Last update date |
Jul 02, 2014 |
| Contact name |
Gregory Doho |
| E-mail(s) |
gdoho@emory.edu
|
| Organization name |
Emory University
|
| Street address |
1365B Clifton Rd NE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL85 |
| Series (1) |
| GSE55243 |
Genomic Microarray of Rat Alveolar Type 2 cells Following Chronic Ethanol Ingestion |
|
