|
| Status |
Public on Feb 25, 2014 |
| Title |
Tie2-Cre,Pfn1fl/fl:Y129F mouse#1 |
| Sample type |
RNA |
| |
|
| Source name |
Tie2-Cre,Pfn1fl/fl:Y129F
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Tie2-Cre,Pfn1fl/fl:Y129F
cell type: tumor-associated endothelial cells
|
| Treatment protocol |
Isolated endothelial cells were treated with glioma-conditioned medium for 12 h.
|
| Growth protocol |
Endothelial cells were isolated from mouse glioma tumors in Tie2-Cre,Pfn1fl/fl:Y129F and Pfn1fl/fl:Y129F mice, by using magnetic-activated cell sorting with an anti-CD105 antibody
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by using TRIzol reagent according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Mouse 2.0 ST Arrays (Affymetrix) and scanned at the Case Western Reserve University Microarray Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RMA expression value derived from Expression Console software
|
| Data processing |
Raw data were processed using Expression console
|
| |
|
| Submission date |
Feb 24, 2014 |
| Last update date |
Feb 25, 2014 |
| Contact name |
Yi Fan |
| E-mail(s) |
fanyi@uphs.upenn.edu
|
| Organization name |
Cleveland Clinic
|
| Street address |
9500 Euclid Avenue
|
| City |
Cleveland |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE55295 |
Role of Pfn-1 phosphorylation in gene expression in glioma-associated endothelial cells |
|
