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Sample GSM1333744 Query DataSets for GSM1333744
Status Public on Feb 25, 2014
Title Pfn1fl/fl:Y129F mouse#7
Sample type RNA
 
Source name Pfn1fl/fl:Y129F
Organism Mus musculus
Characteristics genotype: Pfn1fl/fl:Y129F
cell type: tumor-associated endothelial cells
Treatment protocol Isolated endothelial cells were treated with glioma-conditioned medium for 12 h.
Growth protocol Endothelial cells were isolated from mouse glioma tumors in Tie2-Cre,Pfn1fl/fl:Y129F and Pfn1fl/fl:Y129F mice, by using magnetic-activated cell sorting with an anti-CD105 antibody
Extracted molecule total RNA
Extraction protocol RNA was extracted by using TRIzol reagent according to the manufacturer's instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with GeneChip Mouse 2.0 ST Arrays (Affymetrix) and scanned at the Case Western Reserve University Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RMA expression value derived from Expression Console software
Data processing Raw data were processed using Expression console
 
Submission date Feb 24, 2014
Last update date Feb 25, 2014
Contact name Yi Fan
E-mail(s) fanyi@uphs.upenn.edu
Organization name Cleveland Clinic
Street address 9500 Euclid Avenue
City Cleveland
ZIP/Postal code 44195
Country USA
 
Platform ID GPL16570
Series (1)
GSE55295 Role of Pfn-1 phosphorylation in gene expression in glioma-associated endothelial cells

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
17210850 12.15611
17210852 10.70152
17210855 1950.541
17210869 1677.802
17210883 57.01879
17210887 1053.919
17210904 72.65733
17210912 1571.669
17210947 81.58295
17210953 31.17409
17210984 809.2435
17210994 35.24077
17210996 59.30439
17210998 34.15948
17211000 225.2613
17211004 162.3863
17211023 98.86219
17211033 66.67493
17211043 292.7916
17211066 22.67968

Total number of rows: 41345

Table truncated, full table size 723 Kbytes.




Supplementary file Size Download File type/resource
GSM1333744_166Fox5.CEL.gz 9.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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