|
| Status |
Public on Feb 09, 2015 |
| Title |
KSE_4_23_MT1_04242007_5-1_JC |
| Sample type |
RNA |
| |
|
| Source name |
zebrafish larvae (120 hours post fertilization)
|
| Organism |
Danio rerio |
| Characteristics |
phenotype: UHRF1 mutant
|
| Treatment protocol |
uhrf1 mutant versus wildtype zebrafish larvae at 120 days post fertilization
|
| Growth protocol |
The progeny of uhrf1hi272+/- fish (Sadler et al., 2007) was used in most experiments. Tg(fabp10:hUHRF1-GFP) were outcrossed to uhrf1hi272+/- fish and F2-F5 fish were used.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by using TRIzol reagent (Life Technologies) according to manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
Refer to the Affymetrix System Manual
|
| |
|
| Hybridization protocol |
Refer to the Affymetrix System Manual
|
| Scan protocol |
Refer to the Affymetrix System Manual
|
| Data processing |
Scanned raw data were normalized by using robust multiarray analysis (RMA) algorithm implemented in GenePattern genomic analysis toolkit (www.broadinstitute.org/genepattern).
|
| |
|
| Submission date |
Feb 25, 2014 |
| Last update date |
Feb 09, 2015 |
| Contact name |
Yujin Hoshida |
| E-mail(s) |
Yujin.Hoshida@UTSouthwestern.edu
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE55339 |
Gene expression profiles of uhrf1 mutant zebrafish |
|
