|
| Status |
Public on Oct 27, 2014 |
| Title |
E6 het 4h |
| Sample type |
RNA |
| |
|
| Source name |
T cells 4h stimulated
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6.C-Tg(CMV-cre)1Cgn/J
genotype: Malt1+/-
period of stimulation: 4h
treatment: stimulated
cell type: T cells
|
| Treatment protocol |
Purification and stimulation of T-cells. Total T-cells were purified by negative selection using magnetic beads (Dynal, Invitrogen) against B cells (B220) and myeloid cells (CD11b). CD4+ T-cells were purified using MACS CD4+ or CD4+CD62L+ T Cell isolation kits (Miltenyi). Cells were stimulated with PMA (Adipogen) and ionomycin (Alexis) or with plate-bound anti-CD3 (145-2C11) and anti-CD28 (37.51, both eBioscience) antibodies after pre-coating with anti-Syrian hamster antibodies (F(ab')2 fragment of IgG H+L, rabbit, Jackson ImmunoResearch) at given concentrations and for indicated time points at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol applied for Illumina HiScan
|
| Description |
KO heterozygous
replicate 4
|
| Data processing |
The data were normalised using quantile normalisation with Illumina Genomestudio 2011.1
|
| |
|
| Submission date |
Feb 26, 2014 |
| Last update date |
Oct 27, 2014 |
| Contact name |
Martin Irmler |
| Organization name |
Helmholtz Zentrum München GmbH
|
| Department |
Institute of Experimental Genetics
|
| Lab |
Gene Regulation & Epigenetics
|
| Street address |
Ingolstaedter Landstrasse 1
|
| City |
Neuherberg |
| State/province |
Bayern |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE55360 |
Uncoupling Malt1 threshold function from paracaspase activity results in destructive autoimmune inflammation |
|
