|
Status |
Public on Oct 27, 2014 |
Title |
E6 hom 0h |
Sample type |
RNA |
|
|
Source name |
T cells non-stimulated
|
Organism |
Mus musculus |
Characteristics |
strain: B6.C-Tg(CMV-cre)1Cgn/J genotype: Malt1-/- period of stimulation: 0h treatment: non-stimulated cell type: T cells
|
Treatment protocol |
Purification and stimulation of T-cells. Total T-cells were purified by negative selection using magnetic beads (Dynal, Invitrogen) against B cells (B220) and myeloid cells (CD11b). CD4+ T-cells were purified using MACS CD4+ or CD4+CD62L+ T Cell isolation kits (Miltenyi). Cells were stimulated with PMA (Adipogen) and ionomycin (Alexis) or with plate-bound anti-CD3 (145-2C11) and anti-CD28 (37.51, both eBioscience) antibodies after pre-coating with anti-Syrian hamster antibodies (F(ab')2 fragment of IgG H+L, rabbit, Jackson ImmunoResearch) at given concentrations and for indicated time points at 37°C.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol applied for Illumina HiScan
|
Description |
KO homozygous replicate 4
|
Data processing |
The data were normalised using quantile normalisation with Illumina Genomestudio 2011.1
|
|
|
Submission date |
Feb 26, 2014 |
Last update date |
Oct 27, 2014 |
Contact name |
Martin Irmler |
Organization name |
Helmholtz Zentrum München GmbH
|
Department |
Institute of Experimental Genetics
|
Lab |
Gene Regulation & Epigenetics
|
Street address |
Ingolstaedter Landstrasse 1
|
City |
Neuherberg |
State/province |
Bayern |
ZIP/Postal code |
85764 |
Country |
Germany |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE55360 |
Uncoupling Malt1 threshold function from paracaspase activity results in destructive autoimmune inflammation |
|