|
| Status |
Public on Jul 31, 2014 |
| Title |
1923-2WT-L |
| Sample type |
RNA |
| |
|
| Source name |
Wild type appendicular bones
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: wild type
tissue: entire appendicular skeleton elements such as femora, tibia, humeri
age: embryonic (E) 16.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy kit as per manufacturer's protocol provided with kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAMp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Replicate 3
|
| Data processing |
The data were normalized using quantile normalization with Illumina GUI in R.
|
| |
|
| Submission date |
Mar 04, 2014 |
| Last update date |
Jul 31, 2014 |
| Contact name |
Debabrata Patra |
| E-mail(s) |
patrad@wudosis.wustl.edu
|
| Phone |
314 454 8823
|
| Organization name |
Washington University School of Medicine
|
| Department |
Orthopaedic Surgery
|
| Lab |
BJCIH
|
| Street address |
425 S. Euclid Avenue
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6481 |
| Series (1) |
| GSE55577 |
Genome-wide analysis of cartilage gene expression in response to cartilage-specific ablation of Site-1 protease (Mbtps1) in mice (S1Pcko mice) |
|
