|
Status |
Public on Jul 31, 2014 |
Title |
1927-WT-C-amp#2 |
Sample type |
RNA |
|
|
Source name |
Wild type chondroepiphyseal cartilage
|
Organism |
Mus musculus |
Characteristics |
strain/background: C57BL/6 genotype/variation: wild type tissue: chondroepiphyseal cartilage from most appendicular skeletal elements age: embryonic (E) 16.5
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy kit as per manufacturer's protocol provided with kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAMp kit for Illumina arrays.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Description |
Replicate 2
|
Data processing |
The data were normalized using quantile normalization with Illumina GUI in R.
|
|
|
Submission date |
Mar 04, 2014 |
Last update date |
Jul 31, 2014 |
Contact name |
Debabrata Patra |
E-mail(s) |
patrad@wudosis.wustl.edu
|
Phone |
314 454 8823
|
Organization name |
Washington University School of Medicine
|
Department |
Orthopaedic Surgery
|
Lab |
BJCIH
|
Street address |
425 S. Euclid Avenue
|
City |
St. Louis |
State/province |
MO |
ZIP/Postal code |
63110 |
Country |
USA |
|
|
Platform ID |
GPL6481 |
Series (1) |
GSE55577 |
Genome-wide analysis of cartilage gene expression in response to cartilage-specific ablation of Site-1 protease (Mbtps1) in mice (S1Pcko mice) |
|