|
| Status |
Public on Oct 30, 2014 |
| Title |
Tumor-associated-macrophages_wild type_1 |
| Sample type |
RNA |
| |
|
| Source name |
WT_TS/A tumor_21 day_TAM
|
| Organism |
Mus musculus |
| Characteristics |
strain background: Balb/c
genotype/variation: wild type
age: 8-12 weeks
gender: female
inoculated with: 5x10e6 TS/A cells
tissue harvested: TS/A tumors 21 days after tumor challenge
cell type analyzed: tumor-associated macrophages (TAM)
|
| Treatment protocol |
TAM were lysed 30 min after isolation without additional treatments
|
| Growth protocol |
TAM were isolated from tumors using CD11b MACS beads, adhered in DMEM medium supplemented with 10% FBS for 30 min and lysed for RNA isolation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeasy midi kit (Qiagen, Hilden, Germany) according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Wt2
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 5, from SAS. Gene expression profiling was performed using arrays of Mouse430_2-type from Affymetrix. A Custom CDF Version 12 with entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying RMA.
|
| |
|
| Submission date |
Mar 05, 2014 |
| Last update date |
Oct 30, 2014 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18376 |
| Series (1) |
| GSE55595 |
Endocytic and signalling function of stabilin-1 in tumor associated macrophages |
|
