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Sample GSM1340527 Query DataSets for GSM1340527
Status Public on Mar 06, 2014
Title MEKi 4h biological replicate 1
Sample type RNA
 
Source name SW480 cells
Organism Homo sapiens
Characteristics lineage: colorectal
cell line: SW480
treatment: treated with MEK inhibitor for 4h
Treatment protocol 2.5 million cells were resuspended in 10ml of fresh media in 10cm dishes. The next day, DMSO, TNKS inhibitor, MEKi inhibitor or the combination of both was added for 4hrs and 16hrs
Growth protocol SW480 cells were cultured in 10% FBS RPMI.
Extracted molecule total RNA
Extraction protocol RNeasy Plus kit (qiagen) following the manufacturers instructions
Label biotin
Label protocol Nugen Ovation RNA Amplification System+ WB + FL cDNA Biotin Module V2 (NuGen Lts)
 
Hybridization protocol Affymetrix GeneChip eukaryotic hybridization protocol (Affymetrix Inc.)
Scan protocol GeneChip Scanner 3000 (Affymetrix Inc.)
Description Gene expression data from SW480 cells treated with MEK inhibitor for 4h
Data processing Micorarray Analysis Suite version 5.0 (MAS 5.0) background correction, scaled to 150
 
Submission date Mar 05, 2014
Last update date Mar 06, 2014
Contact name Joshua M Korn
E-mail(s) joshkorn@gmail.com
Organization name Novartis
Street address 250 Mass Ave
City Cambridge
State/province MA
ZIP/Postal code 02141
Country USA
 
Platform ID GPL570
Series (1)
GSE55624 Inhibiting tankyrases sensitizes KRAS mutant cancer cells to MEK inhibitors by FGFR2 feedback signaling

Data table header descriptions
ID_REF
VALUE MAS5 with a target trimmed mean of 150

Data table
ID_REF VALUE
AFFX-BioB-5_at 260.9
AFFX-BioB-M_at 415.9
AFFX-BioB-3_at 332.3
AFFX-BioC-5_at 770.9
AFFX-BioC-3_at 744.4
AFFX-BioDn-5_at 1548.3
AFFX-BioDn-3_at 2005.2
AFFX-CreX-5_at 4047.2
AFFX-CreX-3_at 3720.6
AFFX-DapX-5_at 49.8
AFFX-DapX-M_at 114.3
AFFX-DapX-3_at 246.1
AFFX-LysX-5_at 21.2
AFFX-LysX-M_at 30.6
AFFX-LysX-3_at 46.9
AFFX-PheX-5_at 16.3
AFFX-PheX-M_at 32.1
AFFX-PheX-3_at 52.9
AFFX-ThrX-5_at 17.9
AFFX-ThrX-M_at 15.7

Total number of rows: 54675

Table truncated, full table size 829 Kbytes.




Supplementary file Size Download File type/resource
GSM1340527_NUID-0000-0150-5247.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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