|
| Status |
Public on Mar 06, 2014 |
| Title |
MEKi 4h biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
SW480 cells
|
| Organism |
Homo sapiens |
| Characteristics |
lineage: colorectal
cell line: SW480
treatment: treated with MEK inhibitor for 4h
|
| Treatment protocol |
2.5 million cells were resuspended in 10ml of fresh media in 10cm dishes. The next day, DMSO, TNKS inhibitor, MEKi inhibitor or the combination of both was added for 4hrs and 16hrs
|
| Growth protocol |
SW480 cells were cultured in 10% FBS RPMI.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plus kit (qiagen) following the manufacturers instructions
|
| Label |
biotin
|
| Label protocol |
Nugen Ovation RNA Amplification System+ WB + FL cDNA Biotin Module V2 (NuGen Lts)
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip eukaryotic hybridization protocol (Affymetrix Inc.)
|
| Scan protocol |
GeneChip Scanner 3000 (Affymetrix Inc.)
|
| Description |
Gene expression data from SW480 cells treated with MEK inhibitor for 4h
|
| Data processing |
Micorarray Analysis Suite version 5.0 (MAS 5.0) background correction, scaled to 150
|
| |
|
| Submission date |
Mar 05, 2014 |
| Last update date |
Mar 06, 2014 |
| Contact name |
Joshua M Korn |
| E-mail(s) |
joshkorn@gmail.com
|
| Organization name |
Novartis
|
| Street address |
250 Mass Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02141 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE55624 |
Inhibiting tankyrases sensitizes KRAS mutant cancer cells to MEK inhibitors by FGFR2 feedback signaling |
|
