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Sample GSM1340532 Query DataSets for GSM1340532
Status Public on Mar 06, 2014
Title DMSO 16h biological replicate 2
Sample type RNA
 
Source name SW480 cells
Organism Homo sapiens
Characteristics lineage: colorectal
cell line: SW480
treatment: treated with DMSO for 16h
Treatment protocol 2.5 million cells were resuspended in 10ml of fresh media in 10cm dishes. The next day, DMSO, TNKS inhibitor, MEKi inhibitor or the combination of both was added for 4hrs and 16hrs
Growth protocol SW480 cells were cultured in 10% FBS RPMI.
Extracted molecule total RNA
Extraction protocol RNeasy Plus kit (qiagen) following the manufacturers instructions
Label biotin
Label protocol Nugen Ovation RNA Amplification System+ WB + FL cDNA Biotin Module V2 (NuGen Lts)
 
Hybridization protocol Affymetrix GeneChip eukaryotic hybridization protocol (Affymetrix Inc.)
Scan protocol GeneChip Scanner 3000 (Affymetrix Inc.)
Description Gene expression data from SW480 cells treated with DMSO for 16h
Data processing Micorarray Analysis Suite version 5.0 (MAS 5.0) background correction, scaled to 150
 
Submission date Mar 05, 2014
Last update date Mar 06, 2014
Contact name Joshua M Korn
E-mail(s) joshkorn@gmail.com
Organization name Novartis
Street address 250 Mass Ave
City Cambridge
State/province MA
ZIP/Postal code 02141
Country USA
 
Platform ID GPL570
Series (1)
GSE55624 Inhibiting tankyrases sensitizes KRAS mutant cancer cells to MEK inhibitors by FGFR2 feedback signaling

Data table header descriptions
ID_REF
VALUE MAS5 with a target trimmed mean of 150

Data table
ID_REF VALUE
AFFX-BioB-5_at 268.5
AFFX-BioB-M_at 406.2
AFFX-BioB-3_at 375.8
AFFX-BioC-5_at 801.5
AFFX-BioC-3_at 749.3
AFFX-BioDn-5_at 1690.1
AFFX-BioDn-3_at 2214.2
AFFX-CreX-5_at 4715.8
AFFX-CreX-3_at 4142.6
AFFX-DapX-5_at 39.4
AFFX-DapX-M_at 94.7
AFFX-DapX-3_at 227.7
AFFX-LysX-5_at 15.1
AFFX-LysX-M_at 23.9
AFFX-LysX-3_at 38.8
AFFX-PheX-5_at 10.6
AFFX-PheX-M_at 20.2
AFFX-PheX-3_at 44.5
AFFX-ThrX-5_at 12.1
AFFX-ThrX-M_at 13.2

Total number of rows: 54675

Table truncated, full table size 827 Kbytes.




Supplementary file Size Download File type/resource
GSM1340532_NUID-0000-0150-5243.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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