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Sample GSM1340535 Query DataSets for GSM1340535
Status Public on Mar 06, 2014
Title MEKi 16h biological replicate 1
Sample type RNA
Source name SW480 cells
Organism Homo sapiens
Characteristics lineage: colorectal
cell line: SW480
treatment: treated with MEK inhibitor for 16h
Treatment protocol 2.5 million cells were resuspended in 10ml of fresh media in 10cm dishes. The next day, DMSO, TNKS inhibitor, MEKi inhibitor or the combination of both was added for 4hrs and 16hrs
Growth protocol SW480 cells were cultured in 10% FBS RPMI.
Extracted molecule total RNA
Extraction protocol RNeasy Plus kit (qiagen) following the manufacturers instructions
Label biotin
Label protocol Nugen Ovation RNA Amplification System+ WB + FL cDNA Biotin Module V2 (NuGen Lts)
Hybridization protocol Affymetrix GeneChip eukaryotic hybridization protocol (Affymetrix Inc.)
Scan protocol GeneChip Scanner 3000 (Affymetrix Inc.)
Description Gene expression data from SW480 cells treated with MEK inhibitor for 16h
Data processing Micorarray Analysis Suite version 5.0 (MAS 5.0) background correction, scaled to 150
Submission date Mar 05, 2014
Last update date Mar 06, 2014
Contact name Joshua M Korn
Organization name Novartis
Street address 250 Mass Ave
City Cambridge
State/province MA
ZIP/Postal code 02141
Country USA
Platform ID GPL570
Series (1)
GSE55624 Inhibiting tankyrases sensitizes KRAS mutant cancer cells to MEK inhibitors by FGFR2 feedback signaling

Data table header descriptions
VALUE MAS5 with a target trimmed mean of 150

Data table
AFFX-BioB-5_at 287.4
AFFX-BioB-M_at 462.5
AFFX-BioB-3_at 383.1
AFFX-BioC-5_at 876.1
AFFX-BioC-3_at 755.1
AFFX-BioDn-5_at 1795.4
AFFX-BioDn-3_at 2277.1
AFFX-CreX-5_at 4666.5
AFFX-CreX-3_at 4217
AFFX-DapX-5_at 43.9
AFFX-DapX-M_at 117.4
AFFX-DapX-3_at 263.3
AFFX-LysX-5_at 16.5
AFFX-LysX-M_at 31.7
AFFX-LysX-3_at 45.6
AFFX-PheX-5_at 12.2
AFFX-PheX-M_at 19.4
AFFX-PheX-3_at 38.5
AFFX-ThrX-5_at 13.2
AFFX-ThrX-M_at 15

Total number of rows: 54675

Table truncated, full table size 829 Kbytes.

Supplementary file Size Download File type/resource
GSM1340535_NUID-0000-0150-5248.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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