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Sample GSM1340537 Query DataSets for GSM1340537
Status Public on Mar 06, 2014
Title Combo 16h biological replicate 1
Sample type RNA
 
Source name SW480 cells
Organism Homo sapiens
Characteristics lineage: colorectal
cell line: SW480
treatment: treated with MEK+TNKS inhibitors for 16h
Treatment protocol 2.5 million cells were resuspended in 10ml of fresh media in 10cm dishes. The next day, DMSO, TNKS inhibitor, MEKi inhibitor or the combination of both was added for 4hrs and 16hrs
Growth protocol SW480 cells were cultured in 10% FBS RPMI.
Extracted molecule total RNA
Extraction protocol RNeasy Plus kit (qiagen) following the manufacturers instructions
Label biotin
Label protocol Nugen Ovation RNA Amplification System+ WB + FL cDNA Biotin Module V2 (NuGen Lts)
 
Hybridization protocol Affymetrix GeneChip eukaryotic hybridization protocol (Affymetrix Inc.)
Scan protocol GeneChip Scanner 3000 (Affymetrix Inc.)
Description Gene expression data from SW480 cells treated with MEK+TNKS inhibitors for 16h
Data processing Micorarray Analysis Suite version 5.0 (MAS 5.0) background correction, scaled to 150
 
Submission date Mar 05, 2014
Last update date Mar 06, 2014
Contact name Joshua M Korn
E-mail(s) joshkorn@gmail.com
Organization name Novartis
Street address 250 Mass Ave
City Cambridge
State/province MA
ZIP/Postal code 02141
Country USA
 
Platform ID GPL570
Series (1)
GSE55624 Inhibiting tankyrases sensitizes KRAS mutant cancer cells to MEK inhibitors by FGFR2 feedback signaling

Data table header descriptions
ID_REF
VALUE MAS5 with a target trimmed mean of 150

Data table
ID_REF VALUE
AFFX-BioB-5_at 221.5
AFFX-BioB-M_at 354
AFFX-BioB-3_at 311.2
AFFX-BioC-5_at 655.2
AFFX-BioC-3_at 610
AFFX-BioDn-5_at 1392.4
AFFX-BioDn-3_at 1815.8
AFFX-CreX-5_at 3746.4
AFFX-CreX-3_at 3330.7
AFFX-DapX-5_at 56.9
AFFX-DapX-M_at 113.6
AFFX-DapX-3_at 234.4
AFFX-LysX-5_at 15.6
AFFX-LysX-M_at 28.2
AFFX-LysX-3_at 37
AFFX-PheX-5_at 13.6
AFFX-PheX-M_at 21.9
AFFX-PheX-3_at 42.4
AFFX-ThrX-5_at 14.9
AFFX-ThrX-M_at 17.8

Total number of rows: 54675

Table truncated, full table size 830 Kbytes.




Supplementary file Size Download File type/resource
GSM1340537_NUID-0000-0150-5240.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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