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Sample GSM134717 Query DataSets for GSM134717
Status Public on Sep 10, 2006
Title NAc, biological rep2
Sample type RNA
 
Source name nucleus accumbens
Organism Mus musculus
Characteristics Male C57Bl/6J mouse
12 weeks old at the time of sacrifice
Treatment protocol Mice were briefly anesthetized with isoflurane then rapidly decapitated. Brains were submerged in oxygenated (95% O2-5% CO2) ice-cold sucrose-artificial cerebrospinal fluid solution (in mM: 194 sucrose, 20 NaCl, 4.4 KCl, 2 CaCl2, 1 MgCl2, 1.2 NaH2PO4, 10.0 glucose, and 26.0 NaHCO3) and coronal brain slices (300 um) were made using a vibratome (Leica). Slices were transferred onto a glass Petri dish with a transfer pipette and excess fluid was drained from the area surrounding the tissue. The Petri dish was placed on top of a chilled aluminum block and tissue was brought to a semi-frozen state before punches were taken.
Extracted molecule total RNA
Extraction protocol Stat-60 (Tel-Test) extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Starting with 10 ng of total RNA, cRNA targets were generated using the two-cycle target labeling method.
 
Hybridization protocol Labeled cRNA was purified, and 20 ?g of cRNA was fragmented to a range of 35 to 200 bases in length. Samples were hybridized at 450C for 16 h to Affymetrix mouse430_2 chips GeneChips were washed and stained in the Affymetrix Fluidic
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from nucleus accumbens of mouse
Data processing The data were analyzed with dChip softwareusing invariant-set normalization method.
 
Submission date Sep 05, 2006
Last update date Aug 28, 2018
Contact name Yong Huang
E-mail(s) yh9fj@virginia.edu
Phone (434) 243-0842
Organization name University of Viginia
Department Medicine
Street address 1340 Jefferson Park Ave
City Charlottesville
State/province VA
ZIP/Postal code 22908
Country USA
 
Platform ID GPL1261
Series (1)
GSE5763 Expression data from mouse brain region bed nucleus of the stria terminalis, nucleus accumbens, and dorsal striatum.
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE dChip PM/MM model calculated expression values
ABS_CALL calculated by GCOS v4.0 software; the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
1415670_at 425.878 P
1415671_at 877.674 P
1415672_at 1288.73 P
1415673_at 180.012 P
1415674_a_at 417.669 P
1415675_at 412.527 P
1415676_a_at 1279.78 P
1415677_at 244.447 P
1415678_at 1025.32 P
1415679_at 1109.76 P
1415680_at 262.65 P
1415681_at 385.5 P
1415682_at 292.103 P
1415683_at 815.047 P
1415684_at 132.414 P
1415685_at 158.067 P
1415686_at 482.883 P
1415687_a_at 3698.26 P
1415688_at 822.846 P
1415689_s_at 259.943 P

Total number of rows: 45037

Table truncated, full table size 936 Kbytes.




Supplementary file Size Download File type/resource
GSM134717.CEL.gz 3.6 Mb (ftp)(http) CEL

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