|
| Status |
Public on Mar 14, 2014 |
| Title |
Osteoblasts_Lrp5_Knockout_Day7_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Osteoblasts_Lrp5_Knockout_Day7
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57Bl/6
genotype/variation: Lrp5-/-
cell type: primary calvarial osteoblasts
time point: day 7 of differentiation
|
| Treatment protocol |
Osteoblasts were infected with adenovirus expressing Cre-recombinase or Green fluorescent protein 48 hours prior to inducing osteoblast differentiation.
|
| Growth protocol |
Cultured for 7 days in the presence of 50ug/ml Ascorbic Acid and 10mM Beta-glycerol phosphate to induce osteoblast differentiation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen Rneasy Mini Kit.
|
| Label |
Biotin
|
| Label protocol |
Biotin-labeled cRNA was synthesized by the total prep RNA amplification kit from Ambion (Austin, TX).
|
| |
|
| Hybridization protocol |
cRNA was quantified and normalized to 77 ng/μl, and then 850 ng was hybridized to Beadchips.
|
| Scan protocol |
The hybridized Beadchips were scanned by Illumina BeadScan confocal scanner. cRNA synthesis, hybridization and scanning was performed by the Genome Sciences Laboratory in the Center for Public Health Genomics at the University of Virginia.
|
| Description |
Lrp5_7_KO_2
|
| Data processing |
The expression values were log2 transformed and quantile normalized using the LumiR (v.2.14.2) R package. Matrix non-normalized.txt contains Illumina GenomeStudio (framework 2011.1) generated raw intensity values.
|
| |
|
| Submission date |
Mar 13, 2014 |
| Last update date |
Mar 14, 2014 |
| Contact name |
Charles R Farber |
| E-mail(s) |
crf2s@virginia.edu
|
| Organization name |
University of Virginia
|
| Department |
Center for Public Health Genomics
|
| Street address |
P.O. Box 800717
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE55900 |
Low-density lipoprotein receptor-5 contributes to whole body metabolism by regulating fatty acid metabolism in the osteoblast |
|
