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Sample GSM1348585 Query DataSets for GSM1348585
Status Public on Dec 02, 2014
Title Subject 9 - 1,5h post-meal
Sample type RNA
 
Source name Human skeletal muscle
Organism Homo sapiens
Characteristics tissue: Skeletal muscle (musculus vastus lateralis)
gender: Male
post-meal time: 1,5h
individual: Subject 9
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from skeletal muscle biopsies (~25-30 mg) using the phenol-chloroform extraction method (Tripure RNA Isolation reagent, Roche, Germany), treated with the TurboDNAse kit according to manufacturer protocol (TurboDNAse, Life Technologies, The Netherlands) and quantified by NanoDrop (ND1000, Thermo Scientific, The Netherlands). The quality and integrity of RNA was tested using 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) and all the samples had a RIN score >8.
Label biotin
Label protocol Biotinylated cRNA were prepared using the Illumina TotalPrep-96 RNA Amplification kit
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using Illumina’s Genomestudio v.2011.1
Data processing The data were normalised using quantile normalisation with the lumi package version 2.4.0 in R
 
Submission date Mar 14, 2014
Last update date Dec 02, 2014
Contact name Bruno Guigas
E-mail(s) b.g.a.guigas@lumc.nl
Organization name Leiden University Medical Center
Department Parasitology
Street address P.O.Box 9600, Postzone L40-Q
City Leiden
ZIP/Postal code 2300RC
Country Netherlands
 
Platform ID GPL10558
Series (1)
GSE55924 Regulation of skeletal muscle energy/nutrient-sensing pathways during metabolic adaptation to fasting in healthy humans

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 7.5 0.0
ILMN_2055271 7.3 0.4
ILMN_1736007 7.2 0.7
ILMN_2383229 7.3 0.2
ILMN_1806310 7.2 0.6
ILMN_1779670 7.1 0.7
ILMN_1653355 7.4 0.2
ILMN_1717783 7.0 0.9
ILMN_1705025 7.3 0.4
ILMN_1814316 8.6 0.0
ILMN_2359168 8.6 0.0
ILMN_1731507 7.4 0.2
ILMN_1787689 7.3 0.3
ILMN_3241953 7.5 0.0
ILMN_1745607 9.3 0.0
ILMN_2136495 7.1 0.8
ILMN_1668111 7.3 0.5
ILMN_2295559 7.5 0.1
ILMN_1735045 7.8 0.0
ILMN_1680754 7.5 0.0

Total number of rows: 47323

Table truncated, full table size 972 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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