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Sample GSM1348746 Query DataSets for GSM1348746
Status Public on Mar 15, 2014
Title gWAT treatment, biological rep1
Sample type RNA
 
Source name SVF from gWAT in drug-treated mice
Organism Mus musculus
Characteristics strain: C57Bl/6
tissue: gonadal white adipose tissue (gWAT)
tissue subtype: stromal vascular fraction (SVF)
age: 8-week
Treatment protocol wt C57Bl/6 mice were intraperitoneally (i.p.) injected with CL316243 (1 mg/kg in 0.1 ml) once daily for 3 consecutive days. Stromal vascular fraction (SVF) from gWAT of vehicle- and CL-treated mice were fractioned by a collagenase digestion method. The SVF were served as RNA extraction.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Encore biotin module(4200,Nugen) followed the manufacturer’s instructions to obtain biotin labeled cDNA.
 
Hybridization protocol Array hybridization and wash was performed using GeneChip Hybridization, wash and stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00‐0331‐220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00‐0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
Scan protocol Slides were scanned by GeneChip® Scanner 3000 (Cat#00‐00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
Description treatment:CL316243, 1 mg/kg, 3 days
Data processing Raw data were normalized by RMA algorithm, Expression Console.
 
Submission date Mar 14, 2014
Last update date Mar 15, 2014
Contact name Takahiro Seki
E-mail(s) takahiro.seki@ki.se
Organization name Karolinska Institutet
Street address Nobels vag16
City Stockholm
ZIP/Postal code 17177
Country Sweden
 
Platform ID GPL16570
Series (1)
GSE55934 Expression data from Mus musculus white adipose tissue

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
17210850 2.108022
17210852 2.36804
17210855 7.503522
17210869 8.081923
17210883 2.702151
17210887 6.854614
17210904 2.953899
17210912 7.058642
17210947 2.397214
17210953 2.746434
17210984 8.148567
17210994 2.958609
17210996 2.129865
17210998 2.229147
17211000 5.848961
17211004 6.951401
17211023 3.012926
17211033 3.273068
17211043 6.619691
17211066 2.793233

Total number of rows: 41345

Table truncated, full table size 722 Kbytes.




Supplementary file Size Download File type/resource
GSM1348746_BH13505-5_TS-13_MoGene-2_0-st.CEL.gz 8.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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