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Sample GSM1348814 Query DataSets for GSM1348814
Status Public on Aug 12, 2016
Title Male Knock Out 4
Sample type RNA
 
Source name Mice Heart
Organism Mus musculus
Characteristics strain: C57BL/6
gender: Male
tissue: Heart
age: 18 Weeks
genotype/variation: cardiac-specific estrogen receptor alpha knockout
Treatment protocol No Treatment
Growth protocol Wild type and cardiac-specific ErĪ± knockout, male and female mice of C57BL/6 phenotype were bred and maintained at Washington University School of Medicine animal facility. Mice had free access to regular diet and water.
Extracted molecule total RNA
Extraction protocol RNA was ectracted with Qiagen Total RNA isolation lipid Kit with on-column Dnase 1 treatment in accordance with prescribed protocol provided in the kit. Quality control was performed with Agilient Bioanalyzer
Label Biotin
Label protocol Biotinylated aRNA were prepared with Message Amp total prep amplifciation kit , Life technoligies
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Male Knock out Replicate 4
Data processing The data were normalized using cubic spliine normalization with IlluminaGUI in R
 
Submission date Mar 14, 2014
Last update date Aug 12, 2016
Contact name Kooresh Isaac Shoghi
E-mail(s) shoghik@wustl.edu
Phone 314-362-8990
Organization name Washington University School of Medicine
Department Department of Radiology
Street address 510 South Kingshighway Blvd.
City Saint Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL6885
Series (1)
GSE55936 Network and pathway microarray expression analysis of cardiac-specific estrogen receptor alpha knockout mice

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 6769.152 0
ILMN_2721178 1726.193 0
ILMN_3033922 1088.556 0
ILMN_3092673 4697.833 0
ILMN_2816356 69.20791 0
ILMN_2808939 196.2682 0
ILMN_2634564 340.8458 0
ILMN_2737647 31.56489 0.02631579
ILMN_2734484 340.3575 0
ILMN_2952292 35.6521 0.01754386
ILMN_2699078 -14.73865 0.9273183
ILMN_1213681 1698.481 0
ILMN_2735413 -0.1362754 0.4448622
ILMN_2735415 5.090859 0.2794486
ILMN_2891688 1174.697 0
ILMN_2637698 993.9645 0
ILMN_2674228 178.1517 0
ILMN_2601546 8.654792 0.1779449
ILMN_1230831 12.60765 0.1278196
ILMN_2848071 10.04671 0.1578947

Total number of rows: 25697

Table truncated, full table size 738 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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