|
| Status |
Public on Aug 12, 2016 |
| Title |
Female Knock Out 4 |
| Sample type |
RNA |
| |
|
| Source name |
Mice Heart
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: Female
tissue: Heart
age: 18 Weeks
genotype/variation: cardiac-specific estrogen receptor alpha knockout
|
| Treatment protocol |
No Treatment
|
| Growth protocol |
Wild type and cardiac-specific ErĪ± knockout, male and female mice of C57BL/6 phenotype were bred and maintained at Washington University School of Medicine animal facility. Mice had free access to regular diet and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was ectracted with Qiagen Total RNA isolation lipid Kit with on-column Dnase 1 treatment in accordance with prescribed protocol provided in the kit. Quality control was performed with Agilient Bioanalyzer
|
| Label |
Biotin
|
| Label protocol |
Biotinylated aRNA were prepared with Message Amp total prep amplifciation kit , Life technoligies
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Female Knock out Replicate 4
|
| Data processing |
The data were normalized using cubic spliine normalization with IlluminaGUI in R
|
| |
|
| Submission date |
Mar 14, 2014 |
| Last update date |
Aug 12, 2016 |
| Contact name |
Kooresh Isaac Shoghi |
| E-mail(s) |
shoghik@wustl.edu
|
| Phone |
314-362-8990
|
| Organization name |
Washington University School of Medicine
|
| Department |
Department of Radiology
|
| Street address |
510 South Kingshighway Blvd.
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE55936 |
Network and pathway microarray expression analysis of cardiac-specific estrogen receptor alpha knockout mice |
|
