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Sample GSM1348838 Query DataSets for GSM1348838
Status Public on Jul 01, 2014
Title Control Paxgene blood UofASS 036
Sample type RNA
 
Source name Paxgene venous blood
Organism Homo sapiens
Characteristics diagnosis: Control
tissue: venous blood
Treatment protocol PAXgene tube, frozen -80'C
Growth protocol Venous blood draw into PAXgene tube
Extracted molecule total RNA
Extraction protocol PAXgene blood miRNA kit, Pre-AnalytiX, Quantity and quality of RNA was determined by Nano-Drop (Thermo Fisher) and Agilent 2100 Bioanalyzer (Agilent). Samples required A260/A280 absorbance ratio ≥1.8, A260/A230 ≥1.8, 28S/18S rRNA ratio ≥1.8, and an RNA integrity number ≥8.
Label biotin
Label protocol 200ng total RNA labelled with FlashTag Biotin HSR labeling kits (Affymetrix)
 
Hybridization protocol Affymetrix miRNA GeneChip 3.0 protocol, 45'C 18hr GeneChip Hybridization Oven 645
Scan protocol Affymetrix GCS3000 Gene Array Scanner
Description miRNA expression from human blood cells
Data processing Partek Genomics Suite 6.6, RMA normalization, log2 transformation, hsa-miR filter
 
Submission date Mar 14, 2014
Last update date Jul 01, 2014
Contact name Glen Jickling
E-mail(s) gcjickling@ucdavis.edu
Organization name University of California Davis
Street address 2805 50th St
City Sacramento
State/province CA
ZIP/Postal code 95817
Country USA
 
Platform ID GPL16384
Series (1)
GSE55937 Cerebrovascular blood microRNA

Data table header descriptions
ID_REF
VALUE RMA log2 signal intensity

Data table
ID_REF VALUE
hsa-let-7a-2-star_st 1.05871
hsa-let-7a-star_st 1.2749
hsa-let-7a_st 13.2934
hsa-let-7b-star_st 1.45973
hsa-let-7b_st 12.904
hsa-let-7c_st 11.5161
hsa-let-7d-star_st 3.65278
hsa-let-7d_st 12.8496
hsa-let-7e-star_st 0.993545
hsa-let-7e_st 7.61009
hsa-let-7f-1-star_st 1.23457
hsa-let-7f-2-star_st 0.958932
hsa-let-7f_st 7.75919
hsa-let-7g-star_st 1.07645
hsa-let-7g_st 9.93717
hsa-let-7i-star_st 2.9242
hsa-let-7i_st 11.5785
hsa-miR-100-star_st 1.43336
hsa-miR-100_st 5.72003
hsa-miR-101-star_st 3.00561

Total number of rows: 1733

Table truncated, full table size 42 Kbytes.




Supplementary file Size Download File type/resource
GSM1348838_UofASS_036_miRNA3.CEL.gz 848.1 Kb (ftp)(http) CEL
Processed data included within Sample table

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