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Sample GSM1348845 Query DataSets for GSM1348845
Status Public on Jul 01, 2014
Title Stroke Paxgene blood UCDSS 127
Sample type RNA
 
Source name Paxgene venous blood
Organism Homo sapiens
Characteristics diagnosis: Ischemic Stroke
tissue: venous blood
Treatment protocol PAXgene tube, frozen -80'C
Growth protocol Venous blood draw into PAXgene tube
Extracted molecule total RNA
Extraction protocol PAXgene blood miRNA kit, Pre-AnalytiX, Quantity and quality of RNA was determined by Nano-Drop (Thermo Fisher) and Agilent 2100 Bioanalyzer (Agilent). Samples required A260/A280 absorbance ratio ≥1.8, A260/A230 ≥1.8, 28S/18S rRNA ratio ≥1.8, and an RNA integrity number ≥8.
Label biotin
Label protocol 200ng total RNA labelled with FlashTag Biotin HSR labeling kits (Affymetrix)
 
Hybridization protocol Affymetrix miRNA GeneChip 3.0 protocol, 45'C 18hr GeneChip Hybridization Oven 645
Scan protocol Affymetrix GCS3000 Gene Array Scanner
Description miRNA expression from human blood cells
Data processing Partek Genomics Suite 6.6, RMA normalization, log2 transformation, hsa-miR filter
 
Submission date Mar 14, 2014
Last update date Jul 01, 2014
Contact name Glen Jickling
E-mail(s) gcjickling@ucdavis.edu
Organization name University of California Davis
Street address 2805 50th St
City Sacramento
State/province CA
ZIP/Postal code 95817
Country USA
 
Platform ID GPL16384
Series (1)
GSE55937 Cerebrovascular blood microRNA

Data table header descriptions
ID_REF
VALUE RMA log2 signal intensity

Data table
ID_REF VALUE
hsa-let-7a-2-star_st 1.47751
hsa-let-7a-star_st 1.17758
hsa-let-7a_st 11.8727
hsa-let-7b-star_st 1.55264
hsa-let-7b_st 11.769
hsa-let-7c_st 10.081
hsa-let-7d-star_st 4.36279
hsa-let-7d_st 12.0509
hsa-let-7e-star_st 1.23323
hsa-let-7e_st 5.0407
hsa-let-7f-1-star_st 1.40738
hsa-let-7f-2-star_st 1.3447
hsa-let-7f_st 5.85157
hsa-let-7g-star_st 1.17758
hsa-let-7g_st 8.0647
hsa-let-7i-star_st 3.05317
hsa-let-7i_st 9.37369
hsa-miR-100-star_st 1.6568
hsa-miR-100_st 7.69212
hsa-miR-101-star_st 2.96131

Total number of rows: 1733

Table truncated, full table size 42 Kbytes.




Supplementary file Size Download File type/resource
GSM1348845_UCDSS_127_miRNA3.CEL.gz 849.4 Kb (ftp)(http) CEL
Processed data included within Sample table

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